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文献和实验Protocol for competitive RT-PCR
and sensitive DNA stain. PCR Methods and Applications 4; p. 234-238 Quantitative RT-PCR. Methods and Applications Book 3; Clontech Laboratories, Inc. Technical Tips Online : Nr. 01214: Eferl et al.: Evaluation of different RNA standards
and sensitive DNA stain. PCR Methods and Applications 4; p. 234-238 Quantitative RT-PCR. Methods and Applications Book 3; Clontech Laboratories, Inc. Technical Tips Online : Nr. 01214: Eferl et al.: Evaluation of different RNA standards for reverse
Access RT-PCR System (Promega)
, two-enzyme system provides sensitive, quick, and reproducible analysis of even rare RNAs.RT-PCR reaction componentsRT-PCR Master Mixreaction vol. (uL)Water5AMV 5X buffer5dNTP’s0.525 mM MgSO41Tf1 DNA polymerase0.5AMV-RT0.5Master Mix 12.5per reactionupstream
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