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上海玉博生物科技有限公司
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文献和实验, QC, Canada; Catalog No. 11 277 073 910). Recommended for single FISH. 6. Biotin RNA labeling mix (Roche Applied Science; Catalog No. 11 685 597 910). 7. RNAguard (Amersham Biosciences, Piscataway, NJ, USA; Catalog No. 27-0816-01).
Inverse PCR & Cycle Sequencing
solution (P/N 50611), and the Perkin Elmer ABI BigDye Terminator Cycle Sequencing Ready Reaction Kit (P/N 4303154). The use of other thermal cyclers and sequencing strategies will require different cycling parameters and sequencing protocols.
Radiolabeling of probes for electrophoretic mobility shift assays
Materials: g -32 P ATP (New England Nuclear/Perkin Elmer, CAT# NEG-035C) “Upper strand” oligonucleotide (at 0.5 ug/ul) “Lower strand” oliognucleotide (at 0.25 ug/ul) T4 polynucleotide kinase (New England Biolabs) 10X T4 polynucleotide
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