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文献和实验SANDWICH ELISA FOR DETECTING SECRETE
SANDWICH ELISA FOR DETECTING SECRETE Materials NUNC-Immuno Plate IIF. Horseradish Peroxidase-Streptavidin (Zymed; 43-4323) PBS or Balanced Salt Solution (BSS). Do not use RPMI or any medium that contains biotin.
can thus be semi-quantitated. ECM Cell ELISA (LTI) This is general protocol. Specific antibodies to integrins are added to pelleted cells and allowed to bind to the cell surface. The nonbound antibody is washed away and an enzyme conjugated
Methods to Quantify Basal and Stress-Induced Cortisol Response in Larval Zebrafish
the previously characterized cortisol RIA for adult zebrafish while offering an alternative to the ELISA method. We also provide a methodology for the use of an acute stress paradigm customized for zebrafish larvae, i.e., acute mild
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