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- MBS1183091-0.1mg（E-Coli大肠杆菌）
- 2025年07月12日
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文献和实验Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
incubator. When dry, add cells. Dry remaining coverslips between a folded sheet of whatman paper (dry as separate coverslips). Keep in a sterile tissue culture dish (can store for a year). Do step 4 before use. Can keep 10-20ml aliquots of 1mg/ml
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
sheet of whatman paper (dry as separate coverslips). Keep in a sterile tissue culture dish (can store for a year). Coat with polyamino acid. Coat coverslips in bulk in 10-15ml 1mg/ml PLL (or 500ug/ml polyornithine
one can use a 10X solution of buffered cell culture media.) Add 1 ml of 0.1 M NaOH and mix. 2. Adjust the pH of the solution to 7.4 ± 0.2 by the addition of a few drops of 0.1M HCl or 0.1 M NaOH. The pH of the solution can be monitored by pH paper
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