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- 2025年07月12日
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文献和实验is lost on tips). Rotate in cold room for 1 hr. Spin and wash beads twice with RIPA (discard supernatant), and then once with Histone Wash Buffer. Add 30 µL of Histone Assay solution. Incubate at 37°C for exactly 30 min. Mix
Immunoprecipitation and Immune Complex kinase assay
9) Transfer to a new eppi tube and wash IP 3x with 500 microliters lysis buffer and then 1x with TN by spinning and resuspending. 10) If doing kinase assay, do additional wash in TN and then proceed with kinase assay (below). 11) Pellets can be stored dry
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
buffer with 5% fresh b-ME,boil 2 min,microfuge 4 min,and load less than half (so you can do it again,if necessary). -------------------------------------------------------------------------------- KINASE ASSAY 1)Resuspend pellets in 10 microliters
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