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- BIOCARE MEDICAL
- DC2012-220V
- 2025年07月15日
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文献和实验RNA Purification Protocol for 0.1-2.5 mg of Animal Tissue
temperature. The precipitated protein and DNA will form a tight pellet. 6. Transfer the supernatant to a new sterile 0.5 mL centrifuge tube that containing 100ul of 100% isopropanol. If the RNA yield is expected to be low, add total 1ug Linear
Preparation of total cellular RNA--提取总RNA方法
volume of 2x proteinaseK buffer and 30μl of 20mg/ml ProtK. (Final conc. 0.2mg/ml)5) Incubate 30 minutes at 42℃6) Extract with 1-2 volumes of Phenol CHCl3 . Spin 10min at 8k at 4℃.7) Repeat step 6 if interface is viscous.8) Extract with 3mls of CHCl3 (Chloroform
Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation
I. Preparing fluoresenctly labeled cDNA (probe): To anneal primer, mix 2ug of mRNA or 50-100m g total RNA with 4ug of a regular or anchored oligo-dT primer in a total volume of 15.4 ul:
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