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文献和实验Quantifying 13C-labeling in Free Sugars and Starch by GC-MS
We describe an approach to extract 13 C-labeled sugars (glucose, fructose, maltose, sucrose, myo -inositol as well as glucose from starch) from plant tissues and to analyze their isotopomer distribution by gas chromatography–mass spectrometry
Chemistry of Minor Groove Binder–Oligonucleotide Conjugates
HEPES·HCl (pH 7.2). (1) no DPI, (2) DPI1 , (3) DPI2 , (4) DPI3 . The concentration of each ODN was 2 × 10–6 M and the A/T ratio was 1:1. Curve 5 shows the melting of the DPI2 conjugate alone in the same buffer. Anomalies in the melting curves may stem
of the model species Escherichia coli (Nat Protoc 4:878–892, 2009).
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