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文献和实验Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
of serum, and about 300-400 ml blood can be collected after the interval of 30-40 days. Such selected donors are used for about a year and then new animals are identified. Animals do not need extra care post collection and can act as healthy donors
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
20× Wash Solution, Phosphate‐Saline Concentrate (50 ml per bottle; from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): transfer contents to a graduated cylinder, bring volume up to 1000 ml with distilled or deionized water
Metaphase chromosome preparation Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best.-Nr. 295892) cell cuture flask Phythemaglutinin, PHA-L (Seromed, M
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