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上海玉博生物科技有限公司
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文献和实验Clonality - X Chromosome Inactivation Assay
(Life Technologies) 10X PCR buffer (100 mM Tris-HCL pH 8.3, 500 mM KCL, 15 mM MgCl2, 0,01 % w/v gelatin, autoclaved) (Perkin Elmer) dNTP, each 10 mM (Perkin Elmer) 7-deaza dGTP, 10 mM (Boehringer Mannheim) DMSO, minimum 99.5%, GC (Sigma
Sample preparation (analytical gels)
containing urea (8 M), CHAPS (4 % w/v), DTE (65 mM), Tris (40 mM) and a trace of bromophenol blue. Hundred μl of the final diluted kidney cell sample was loaded onto the IPG gel strip. LIVER Protocole 1: Five frozen slices (20 μm x 5 mm x 10 mm
Sample preparation (preparative gels)
(8 M), CHAPS (4 % w/v), DTE (65 mM), Resolytes 3.5-10 (2 % v/v) and a trace of bromophenol blue. Five hundred µl of the final diluted colon epithelial cell sample was used for in-gel sample rehydration. CSF (cerebrospinal fluid): An aliquot
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