Cygnus特约代理商|F117-PA Anti-E. co

Purification of dnEBNA-1/Soft from E. coli BL21 LysS

to each 1ml fraction.Save 50μl of each fraction as Elution 1,2,3,4,5 (6,7,8,9,10). Run 2 identical SDS-PAGE gels with all bold samples above,protein size marker,and concentration standards (BSA 0.1μg,1μg,10μg)or protein positive for Soft-tag and/or EBNA-1 d

Purification Of Anti Peptide Antibod

, 20mM TrisCl pH 7.4, 0.2% Triton- X-100. Wash 5 col vols TBS Elute with 0.15 M NaCl, 0.2 M Glycine-HCl pH 2.0. Collect 1 ml fraction, with each tube containing 0.1ml of 2 M TrisCl pH 8.5 Wash with TBS until pH is reequillibrated

（共享）ELISA原理与实验方法

, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS