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上海玉博生物科技有限公司
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文献和实验O and is stored in aliquots at −20 °C. CK solution It comprises 4 mg ml−1 creatine kinase (=3,200 U ml−1 ) in H2 O and is stored in aliquots at −20 °C. Glucose solution
Molecular Investigation of LGMD2B-Haplotype Analysis and Mutation Screening
B have proximal muscle weakness with onset in the late teens, and highly elevated serum creatine kinase levels: The progression of the disease is generally slow. Muscle CT scanning in some patients indicates involvement of the distal muscles
In vitro transcription with yeast nuclear extract
buffer 0.63 microliters 0.1 M DTT 3 microliters phospho creatine (64 mg/ml pH 7.5) 0.1 microliter creatine phospho kinase 1 microliter NTP mix (10 mM each ATP, CTP, GTP, UTP) 150 ng plasmid DNA template(0.125 microliters 10% NP40 can be added
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