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上海玉博生物科技有限公司
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文献和实验kb) 1) Add 0.5 x volume of Buffer P3 and 1 x volume of absolute ethanol (room temperature, 96-100% ) to the DNA solution and vortex to mix well. For example, 100 ul of DNA Solution, add 50 ul of Buffer P3 and 100 ul of absolute ethanol
"Glassing Out" DNA Using the Geneclean Kit
up and down. Incubate the tube in a 50 degree water bath for 3 minutes.\ The DNA does not bind to the silica matrix in the absence of salts and is eluted during this step. 13. Spin for 30 seconds in a microcentrifuge to make a solid pellet. Carefully remove 20 ul
with SPM one more time by repeating step 14-15. 17. After remove the supernatant, air dry the magnetic beads by invert the tube on a absorbent paper for 15 minutes. Remove any residue liquid from tube with pipettor. 18. Add 50-100 ul Elution
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