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文献和实验Identification of the Antigen Content of Electroimmunoprecipitates
methods, e.g., when analyzing complex antigen mixtures. The great advantage of gel immunoprecipitation approaches is that each immunoprecipitate contains antigen in a pure form and that the precipitate is separated by position, shape, and size
and re-hydrated, drying out will cause non-specific antibody binding and therefore high background staining. ii) 3 minutes is only suggested as a starting point antigen retrieval time. Less than 3 minutes may leave the antigens under retrieved, leading to weak
The Use of Adjuvants in Experimental Vaccines: III. Liposomes
being a slower release of the antigen. There is not space here to record the many combinations that could be used; the preparation of a pure sample of phosphatidylcholine (ovo-lecithin) and positively and negatively charged liposomes will be given as examples.
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