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Active Protease Mapping in 2DE Gels

Proteases act as the molecular mediators of many vital biological processes. To understand the function of each protease, it needs to be separated from other proteins and characterized in its natural, biologically active form. In the method

Adenovirus Protease

. GVQSLKRRRCF. The peptide is presumed to regulate enzyme activity in vivo during virus infection. It is bound to C104 on the enzyme via a disulphide bridge (9 ). Mutational analysis and X-ray chrystallography identified the active-site triad as H54-C122-E71

Identifying and Characterizing HIV Protease Inhibitors

HIV protease catalyzes the hydrolysis of specific peptide bonds of viral polyproteins, thus processing these polyproteins into their active components. These protein processing reactions are requisite for viral replication. Therefore, the HIV