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文献和实验they may be split 1:4 into fresh media. (Fungizone can be omitted from this point onwards). 2. Stage I - Preparation of collagen I from rat tails Note: Protocol for approximately 12-14 adolescent (fresh or frozen) rat tails 1) Prepare rat
(Sigma # P7280) Prepare frozen stock in ddH2O @ 100 μg/ml Freshly dilute to 25 μg/ml and coat at 5 μg/cm2 (5 ml/25 cm2) Coat overnight at 37 °C. Wash with sterile PBS 2) Collagen, Type I Rat Tail (Sigma # C7661) Prepare 4 °C stock in 0.25
Mouse Strains and Genetic Nomenclature
strain. It is important to note that genotyping requires many polymorphic DNA markers only for the first back‐cross progenies. Once a marker is characterized homozygous, it is no longer necessary to type it in the forthcoming generations. The y axis
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