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- 2025年07月14日
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文献和实验High Throughput Isolation Of PCR Products Using ChargeSwitch® PCR Clean-Up
elution buffer (see figure below). Purified DNA elutes instantly into this elution buffer. 实验试剂 The components supplied in the ChargeSwitch® PCR Clean-Up Kit are listed below. The reagents supplied are sufficient to perform
Silica Suspension: add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica and resuspend in 2 vol H2O Silica Wash Solution: 50 mM NaCl,10 mM Tris 7.5,2.5 mM EDTA,50% Ethanol 6 M NaI note
Using HTR Reagent to Clean Up DNA Samples with PCR Inhibitors
Important: HTR reagent must be throughly suspended before being dispense from bottle. Tip: Use 1ml pipettor and cut off the end of 1ml tip to make it easier for pipetting the HTR reagent. 2. Incubate at room temperature for 2 minutes.
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