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文献和实验96 Well Mating Protocol platewise
the recombinase driven, temperature sensitive rhamnose inducible PrhaB-gbexo promoter. This promoter is inhibited by glucose. This strain also contains an arabinose inducible promoter paraBAD controlling expression of the I-sceI gene. Induction of I-SceI gene
96 Well Mating Protocol platewise
: Recipient pMSCV: PheS gene flanked with 2-Isce-I site and homologous sites for recombination, 10.6 kb. Carbenicillin (amp) resistant. Recipient Strain BW28705I/pML300: pML300 plasmid carries the recombinase driven, temperature sensitive rhamnose inducible
96 Well Mating Protocol platewise
BW28705I/pML300: pML300 plasmid carries the recombinase driven, temperature sensitive rhamnose inducible PrhaB-gbexo promoter. This promoter is inhibited by glucose. This strain also contains an arabinose inducible promoter paraBAD controlling
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