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文献和实验In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
Basic Protocol 1: Primary Culture of Mouse Mesencephalic Neural Crest Cells Support Protocol 1: Preparation of Chick Embryo Extract (CEE) Reagents and Solutions Commentary
In Situ Hybridization Protocols
and extract with chloroform : isoamyl alcohol. Precipitate upper layer with 1/9 volume of 3M NaOAc and 2 volume ethanol at -80deg.C. Spin down at 4deg.C for 15 minutes. Wash pellet with 70% EtOH and spin again. Resuspend pellet in 20ul of RNAse-free TE
Anderson Lab In Situ Hybridization Protocols
with ice cold acetone and spin down again. Spread pellet out on filter paper, let it dry thoroughly and grind into a fine powder. Store at 4deg.C. To produce 2ml of pre-absorbed antibody: Weigh out 3mg embryo powder and mix
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