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上海玉博生物科技有限公司
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
diiferent blades for each part of the tumor. Overlay with 20 ul mineral oil. Close cap and seal with parafilm. DIGESTION: Incubate overnight for shaking at 120 rpm, 55 C. Day 2: Add 0.3 ul fresh conc Proteinase K (20 mg/ml stock
James Hardwick CNBr Cleavage Procedure
Jagow, G. (1987). Anal. Biochem. 166, 368-379. Acrylamide:Bis ratio for resolving gel from Kunxin's old protocol. Luo, K., and Sefton, B. M. (1990) Transfer of proteins to membranes facilitates both cyanogen bromide cleavage
L-glutamine - - 2mM 2mM - 200mM B-27 - - - 1× 1× 1× N2 - - - 1× 1× 1× HEPES - - - 10mM 10mM - BSA(v/v) - - - - - 0.05% 抗坏血酸 - - - - - 50 µg
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