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上海玉博生物科技有限公司
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文献和实验Pre-Cast Gels for Low-Throughput Nucleic Acid Analysis
-Gel® gel may vary between 15-17 minutes for double-comb gels and 30-33 minutes for single-comb gels. 3) Current through the E-Gel® gel automatically shuts off at the end of each run. The E-Gel® PowerBase™ v.4 signals the end of the run
-filled end.Mutagenesis protocols are provided for both double-stranded DNA (dsDNA ) and single-stranded DNA (ssDNA ). The mutagenesis reaction is initially transformed into a repair minus strain of E. coli (ES1301 mut S) to avoid selection
. The adjacent sequencing primer binding site is protected from digestion by a 4-base 3´ overhang restriction site or by an alpha-phosphorothioate-filled end. Mutagenesis protocols are provided for both double-stranded DNA (dsDNA ) and single-stranded DNA
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