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上海玉博生物科技有限公司
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
centrifugation (3000-5000 x g (6600 rpm) for 1 min)), and remove supernatant (use gel loading tips to completely remove supernatant). (23) Add 1 ml of ice-cold Low Salt Immune Complex Wash Buffer (EZ ChIP kit). Resuspend the packed agarose beads by gentle
Modified Eberwine (antisense) RNA Amplification Protocol
not to spill. Mix by pipeting 5-10X, then transfer slurry to Phase lock gel tubes. Spin 5 minutes maximum speed (15k x g) at room temperature. Transfer aqueous phase to RNase free 1.5 ml eppendorf tube. Add 70 uL 7.5M ammonium acetate (in DEPC water
Steady State ATPase Assays Coupled Enzyme System
. 5. Make Tris-MgCl2 -DTT mix for all assays. Tris-MgCl2 -DTT mix = (n = # of assays + 1) n x 20 µL 0.5 M Tris-OAc, pH 7.5 20 µL 10 mM MgCl2 20 µL 10 mM DTT 70 µL DDW
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