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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
Direct Immunofluorescence Labeling
Recipes Fluorochrome-Conjugated Antibodies: Test antibody: Mouse monoclonal conjugated to FITC, PE, PerCP or PE-CY5 Tandem (tradenames: Cy-Chrome or Tri-Color). If you''re planning to label cells with 2 or more antibodies simultaneously
them individually with different fluorochrome conjugated secondary antibodies? (FITC-conjugated anti-Mouse IgG 1 will label both!) The biotin-avidin system may provide an answer, if one antibody can be biotinylated: label with the non-biotinylated mouse antibody
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