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文献和实验Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
β-mercaptoethanol, 1000X Gibco® Mouse Embryonic Fibroblasts (Irradiated) Basic Fibroblast Growth Factor (bFGF) GlutaMAX™-I (100X) Collagenase Type IV for enzymatic passaging or StemPro® EZPassage™ Tool for mechanical
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
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