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文献和实验Procedures for In Vitro Production of Bovine Embryos
manufacturer’s indications for expiration date. Store at 4°C.Stock 2: Na pyruvate. Dissolve 0.220 g sodium pyruvate in 100 ml water. Sterile-filter into an aluminum-foil wrapped 100 ml bottle and store at 4°C for 1 mo.Stock 3: Bovine Steer Serum (BSS
Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
flasks at approximately 1.3x104 cells/cm2 in Dulbeccos Modified Eagles Medium (DMEM) with 10% Fetal Bovine Serum (FBS). Culture cells for two days in an incubator at 37 °C and 5% CO2 . 2) Make fluorescent g-actin stock solution
(NaKC4H4O6• 4H2O)ReagentsLowry stock reagent49 ml Lowry A0.5 ml Lowry B0.5 ml Lowry CFolin's Reagent: Phenol reagent - 2N (Folin - Ciocalteau reagent).Dilute 1:1 in diH2O before use.Standard: Bovine serum albumin (BSA),lysozyme or other protein
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