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文献和实验Procedures for In Vitro Production of Bovine Embryos
manufacturer’s indications for expiration date. Store at 4°C.Stock 2: Na pyruvate. Dissolve 0.220 g sodium pyruvate in 100 ml water. Sterile-filter into an aluminum-foil wrapped 100 ml bottle and store at 4°C for 1 mo.Stock 3: Bovine Steer Serum (BSS
(NaKC4H4O6• 4H2O)ReagentsLowry stock reagent49 ml Lowry A0.5 ml Lowry B0.5 ml Lowry CFolin's Reagent: Phenol reagent - 2N (Folin - Ciocalteau reagent).Dilute 1:1 in diH2O before use.Standard: Bovine serum albumin (BSA),lysozyme or other protein
, the standard should be similar to the unknown. For example, if one is measuring IgG concentrations, an immunoglobulin standard would be ideal. For serum, use bovine serum albumin as a standard since albumin is a major component of serum. An easy
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