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文献和实验Replication timing by density transfer
. Spin at 50 000 rpm for 18 hr, 28 000 rpm for 3.5 hr, no brake (i.e., deceleration=0) at either step (use dual program). 11. Fractionate the gradients (10 drops per fraction, collected in multi-well microtitre plates), read the refractive index
Detection of Apoptosis in Paraffin-embedded tissues by ISEL (In Situ End Labeling)
from the same block provide the best samples for the titration. SAMPLE dUTP dilution POL I dilution DIGESTION 1A 1:100 1:200 NONE 2A 1:250 1:200
3 exer ts dose dependent dual effects on APL cells. Blood,1997;89:3345-3353 [18] Wang XM, Wang XF, Li J, Evers BM. Effects of 5 azacytidine and butyra te on differentiation and apoptosis of hepatic cancer cell lines.Ann Surg, 1998;227:922-931 [19] Sun
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