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文献和实验” suppressors of nuclease-minus mutations. Genetics 110? 539-555. 25. Petrounia, I. P. and Arnold, F H. (2000) Designed evolution of enzymatic proper-ties. Curr, Opin. BiotechnoL 1 1 , 325-330. 26. Krebber, A. , Bornhauser, S.
A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
DNA, and purified polymerase chain reaction (PCR) products, which are usually subcloned, are sequenced. Bisulfite conversion is so powerful that it has been paired with numerous techniques other than traditional sequencing, including
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
(CAV) DNA in a dilution series: the PCR1 just detects 1000 template molecules; PCR2 amplifies 1 template molecule (Soiné C, Watson SK, Rybicki EP, Lucio B, Nordgren RM, Parrish CR, Schat KA (1993) Avian Dis 37: 467-476)。 Labelling of PCR products
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