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文献和实验to ensure NP-40 has dissolved) PBS: 130 mM NaCl, 2 mM KCl. 8 mM Na2HPO4, 2 mM KH2PO4 Make a 10X stock Night before: Make 600 ml of 1X PBS 0.1X PBS 0.1X PBS, 8% (w/w) ultrapure sucrose 8% (w/w) ultrapure
4 Make a 10X stock Night before: Make 600 ml of 1X PBS 0.1X PBS 0.1X PBS, 8% (w/w) ultrapure sucrose 8% (w/w) ultrapure sucrose LB (w/o BME): add 280 ul BME before use LB + 0.5% NP-40 (w/o BME): add 280
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
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