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文献和实验of gels containing the nonionic detergent Triton X?100, referred to as Triton/acetic acid/urea (TAU) polyacrylamide gels, for analysis of histones. Also included are support protocols detailing several accessory techniques: assembly of gel plates
/µL) 0.5 µL final enzyme amount = 1.75 U Template (cDNA) 1.0 µL Total 50.0 µL
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
depends on thesignalling pathway being analysed. For example, inhibition byphosphorylation (following growth factor stimulation of cells)can be monitored by: 1. Immunoprecipitation of total cellular GSK3 and assay (seeSections
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