tedpella特约代理商|16074 Copper Con
ductive Tape, Double Sided Adhesive, 12.7mm W x 16.4m L each- ￥3000
- eylabs
- L-7901-5
- 2025年07月15日
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上海玉博生物科技有限公司
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文献和实验4 ul of fly supernatant to 16 ul of 1.25X NdeII buffer (125 mM Tris-HCl pH 7.6, 12.5 mM MgCl[2], 188 mM NaCl, 1.25 mM DTT). Add 0.5 ul of NdeII (BRL) and incubate at room temp. for 15 min. Inactivate the enzyme by heating to 65 C for 15 min.
Add 4 ul of fly supernatant to 16 ul of 1.25X NdeII buffer (125 mM Tris-HCl pH 7.6, 12.5 mM MgCl[2], 188 mM NaCl, 1.25 mM DTT). Add 0.5 ul of NdeII (BRL) and incubate at room temp. for 15 min. Inactivate the enzyme by heating to 65 C for 15 min
. After electrophoresis, remove the buffer wells, the tape, and pry the gel plates apart. The gel should adhere to back plate. Blot the gel to a 40 cm X 20 cm sheet of 3MM Whatman paper, cover with plastic wrap, and dry on a Hoefer gel dryer for 25 minutes at 80degC. 11
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