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文献和实验Assays for Transcription Factor Activity
Most transcription activator proteins have three important features that can be probed at the molecular level: They bind to specific sequences near promoters, they can be interconverted between active and inactive forms by covalent or noncovalent
Assaying Transcription Factor Stability
Similar to the activities of transcription factors (TFs) in other eukaryotes, activities of many plant TFs are determined via regulated proteolysis by the ubiquitin/26S proteasome system. Thus, to fully understand the function of a TF
Transcription Factor Sequestration by Polyglutamine Proteins
In polyglutamine diseases including Huntington’s disease, the causative gene products containing expanded polyglutamine form nuclear aggregates in neurons. Recent studies have identified several transcriptional factors, which interact
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