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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Intracellular Immunofluorescent Staining for Flow Cytometry
Right: Mouse splenocytes were stimulated for 3 days with IL-2. Cells were surface stained with APC CD49d (DX5) (Cat. No. 17-5971 ) and subsequently stained intracellularly with PE GB11. Human IL-17 (eBio64CAP17): Human
Native Chromatin Preparation and Illumina/Solexa Library Construction
pellets in 40 µL of T cell isolation buffer per 107 cells. 8. Add 10 µL of biotin-antibody cocktail (from T cell isolation kit) per 107 cells. The biotin-antibody cocktail contains monoclonal antibodies against CD14, CD16, CD19
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