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FFPE Direct PCR Kit

实验步骤   1. Protocol for FFPE Extraction    1) cut a approximately 10 mg FFPE sample into a 2 ml collection tube or suitable vessel, cut off the Samples tiny and remove the paraffin wax as far as possible.   

DIRECT ELISA USING FLUORESCENT SUBSTRATE PROTOCOL

: TBS 250 μl/well; 3x 30 seconds.8. Amplify signal by adding 100 μl/well AttoPhos Fluorescent substrate system, for 5-10 min at RT. 36 mg ofAttoPhos substrate should be mixed with 60 ml of AttoPhos buffer 24 hours prior to use. Make sure well isclean

Basic Method for Direct Immunofluorescence Labeling

immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling. We strongly recommend direct labeling if you're planning to simultaneously label cells