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文献和实验Endothelial Cell Systems - Instructions For Use
EGM ®-2 BulletKit® CC-3162 (or) EGM® BulletKit® CC-3124 HCAEC HIAEC HMVEC-d Neo HMVEC-d Ad HMVEC-L UtMVEC-Myo Coronary Artery Iliac Artery Dermal - Neonatal Dermal - Adult Lung Uterine-Myometrial EGM ®-2 MV BulletKit®
up the fractionation. Ficoll cushion: 20% (w/w) Ficoll (MW 400,000) in 1X PE + 0.1% NP-40 Make up PE + 0.1% NP-40 (no BME). Weigh out 10g Ficoll. Add PE + 0.1 % NP-40 till total weight = 50 grams. Stir at RT for several hours
ul BME before use and put all these buffers in coldroom Sucrose Gradients : Use ultrapure sucrose 20% (w/w) and 62.5% (w/w) sucrose in 1x PE + 0.1% TX-100. Make 100 grams of each as follows: Weigh out sucrose
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