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文献和实验PREPARATION OF PROTEIN A SEPHAROSE CL 4B BEADS
Swelling and Storage1.Resuspend .2 gm of beads (= 1.0 ml swelled bead volume)in 40 ml of distilled water.Let swell for at least 2 hours.2.Wash beads twice in (10 ml each wash).Spin in clinical or table top fuge.Do not exceed 2K rpm.3.Resuspend
Sepharose 4B-CL Chromatography BAC DNA Purification for Transgenic Production
Sepharose 4B-CL Chromatography BAC DNA Purification for Transgenic Production This method was contributed by Wes Dunnick at the University of Michigan Medical School in Ann Arbor, Michigan. The approach is to prepare the DNA
Purification of Mitochondrial Uracil-DNA Glycosylase Using Ugi-Sepharose Affinity Chromatography
) protein as an immobilized ligand on Sepharose 4B beads for purification of mammalian mitochondrial uracil-DNA glycosylase.
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