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文献和实验Detection of Mycoplasma by Culture
Aim Detection of mycoplasma by culture is the reference method of detection and has a theoretical level of detection of 1 colony-forming unit (cfu). However there are some strains of mycoplasma that are non-cultivable (certain strains
Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
of culture. This step also improves sensitivity by increasing the surface area upon which mycoplasma can adhere. Like detection by culture, DNA staining methods are suitable for the detection of mycoplasma from cell cultures or cell culture reagents.
Subculture of Suspension Cell Lines
) Mycoplasma Pig Agar broths (in 1.8ml aliquots) M. orale NCTC* 10112 M. pneumoniae NCTC* 10119 Equipment Personal protective equipment (sterile gloves, laboratory coat, safety visor) Waterbath set to 37ºC Microbiological safety cabinet at appropriate
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