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Phosphoamino acid analysis:Mark Kamps's method

.The blotters should be quite damp,but not soaking wet. 10.Rotate 90° counter clockwise.Electrophorese at pH 3.5,1.6 KV,13 min in the second dimension. The above electrophoresis times are appropriate when you are using a Salk/TVL/MBVL type electrophoresis rig

啤酒酵母(S. cerevisiae)mRNA的提取

off the EtOH and let pellet air dry （or speed vac dry on low heat）. 14. Resuspend in 15-20 ul RNase-free water. 15. Spin down for 30 seconds and quantitate a 1:100 dilution at 260nm. Note: Typically yields should be no greater than 2% of the total RNA input

Analysis of the Protein S Gene in Protein S Deficiency

Protein S (PS) is a 71-kDa vitamin K-dependent glycoprotein first identified in human plasma by DiScipio and colleagues in 1977 (1 ), a year after the discovery of the anticoagulant protein C (PC) (2 ,3 ). A few years later, Walker