merck millipore特约代理商|391334-25

Extraction of DNA using DNAzol® Reagent

实验试剂   1. 100% ethanol 2. 75% ethanol 3. 8 N NaOH   实验步骤   Instructions for Use 1. Lysis/Homogenization 1 ml DNAzol® Reagent 25-50

Technovit® 9100 Methyl Methacrylate

Tris buffer - 0.1 mol/l, pH 9.4 50 ml Fast Blue Solution 25 mg Naphthol-AS-Bi-phosphate dissolved in 0.5 ml dimethyl sulphoxide (DMSO) / Triton X-100 Acid Phosphatase Substrate / Reaction Mixture 50 ml acetate buffer - 0.1 mol/l

Analysis of Proteins using Small Format 2D Gel Electrophoresis

times can be made to use the protocol with other virus-host cell systems. Between 2.2 x 10^7 and 7.1 x 10^8 TCID50 of virus are inoculated onto confluent HEp-2C cell monolayers prepared in 30mm petri dishes. After a 30 minute adsorption at room