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文献和实验1. Migration of marker dyes in native polyacrylamide non-denaturing gels Gel % Bromophenol blue (BP) Xylene cyanole (XC) 3.5 100 460 5.0 65 260 8.0 45 160 12.0 20 70 20.0
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Procedure A. Restriction Digest 1. Cut 10 to 15 μg of CsCl-purified plasmid DNA containing the fragment of interest with 2-fold excess of Restriction Enzyme for 2 hours (see Hint
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS This is a four day procedure so it's best to start on Monday or Tuesday. CASE SELECTION: H&E stained thin sections are first reviewed by a pathologist, and areas
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