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- QIAGEN凯杰
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- 2025年07月14日
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上海玉博生物科技有限公司
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
Miniprep/Kit-free high-throughput protocol
.--Kathleen) and yields DNA that is clean enough for sequencing or for use as a PCR template. Protocol Transfer 1.5 mL of an overnight culture containing your plasmid to an eppendorf tube and spin at 5000 rpm for 5 min in a tabletop centrifuge
Procedure for GenEluteTM Plasmid Miniprep Kit（质粒小量提取）
Note All centrifugation speeds are given in units of g.Please refer to Table 1 for information on vonverting g -force to rpm.If centrifuges/rotors for the required g -forces are not available,use the maximum g -force possible and increase the spin
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