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文献和实验Chromatin Immunoprecipitation (ChIP)
The first chromatin immunoprecipitation (ChIP) assay was developed by Gilmour and Lis (1984, 1985, 1986) as a technique for monitoring the association of RNA polymerase II with transcribed and poised genes in Escherichia coli and Drosophila . The use
A method for the photocrosslinking of proteins to DNA in purified complexes is described. It makes use of the juxtaposition of a limited number of photoreactive nucleotides with a limited number of radiolabeled nucleotides at a specific
Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
polymerase II (Pol II) chromatin immunoprecipitation (ChIP) is a simpler alternative to determine the transcription rate of genes. Moreover, this approach provides more information about the transcriptional regulation of a gene than nuclear run-on. The power
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