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文献和实验V294.PART II，Chapter 2 Boyden法体外趋化试验
-streptomycin and allow them to grow until 50–70%confluency, which usually takes 18 to 24 h. 2. Remove the medium and wash the cells twice with Versene. Add 1 mL of Versenecontaining 0.05% trypsin and allow the culture to stand at 37°C for 10 to 15 min
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
volume withdistilled water. 6. Destaining solution: 30% (v/v) methanol and 10% (v/v) acetic acid. 7. Gel drying solution: 50% (v/v) methanol and 10% (v/v) acetic acid. 8. Cellophane wrap. 2.5. Immunoblotting 1. Mini-gel transfer apparatus to match
Analysis of Proteins using Small Format 2D Gel Electrophoresis
twice with PBS-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck
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