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文献和实验Endothelial Cell Systems - Instructions For Use
Heparin 10 ml FBS (Fetal Bovine Serum) 2% EGM-2, 25 mls in EGM-2-MV 5% 0.5 ml Gentamicin, Amphotercin 4. ReagentPack™ (CC-5034) contains one 100 ml bottle of each of the following subculture reagents: HEPES Buffered Saline Solution (HEPES-BSS) (CC
Native Chromatin Preparation and Illumina/Solexa Library Construction
pellets in 40 µL of T cell isolation buffer per 107 cells. 8. Add 10 µL of biotin-antibody cocktail (from T cell isolation kit) per 107 cells. The biotin-antibody cocktail contains monoclonal antibodies against CD14, CD16, CD
Midi/Maxi Kit. 16. Standard gel electrophoresis reagents and apparatus. 2.2 Cell Culture and Processing Reagents 1. Cell line: human embryonic kidney (HEK) 293FT cell line. 2. Dulbecco’s Modifi ed Eagle’s Medium (DMEM). 3. 10 % fetal
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