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文献和实验Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
a protocol for performing digital gene expression profiling on the Illumina Genome Analyzer sequencing platform. Tag sequencing (Tag?seq) is an implementation of the LongSAGE protocol on the Illumina sequencing platform that increases utility while reducing
Strand-Specific RNA-seq Applied to Malaria Samples
-seq has the power to discover new transcripts and splicing variants, single-nucleotide variations, fusion genes, and mRNA level-based expression profiles. Preparing RNA-seq libraries can be delicate and usually obligates buying expensive kits that require large
SynthesisPCR模板法合成双链短RNA（PCR template method for dsRNA）
——[from Carthew/Clemens/Tuschl] Purpose – a method for synthesis of iRNA which allows both RNA strands to be generated simultaneously from a PCR fragment which contains a T7 promoter on each end. Background
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