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上海玉博生物科技有限公司
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文献和实验blood into 1,5 eppendorf tubes with ACD (acid-citrate-dextrose; Sigma C-3821) = 1 vol of ACD : 3 vol of blood : 6. 6 vol of NaN 3 0,05%-PBS Cetrifuge at 216-220g , 5-7min , 10ѓC Take & save at 4ѓC PRP (platelet-rich plasma )
blood into 1,5 eppendorf tubes with ACD (acid-citrate-dextrose; Sigma C-3821) = 1 vol of ACD : 3 vol of blood : 6. 6 vol of NaN 3 0,05%-PBS Cetrifuge at 216-220g , 5-7min , 10ѓC Take & save at 4ѓC PRP (platelet-rich plasma )
Sample preparation (analytical gels)
containing urea (8 M), CHAPS (4% w/v), Tris (40 mM), DTE (65 mM) and a trace of bromophenol blue. Sixty μl (45 μg) of the final diluted lymphoma sample was loaded on the first dimensional separation. PLASMA: An aliquot of 6.25 μl of human plasma was mixed
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