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Dynabeads® mRNA DIRECT Kit

实验原理   The isolation protocol relies on base pairing between the polyA residues at the 3’ end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads® . Other RNA species lacking a polyA

Mag-Bind® mRNA Protocol (Standard Protocol)

capable of 12,000 x g and 2-8°C 实验步骤   Total RNA Isolation: 1. Homogenization and lysis of samples: follow either method below.    1) Tissue Samples Homogenize tissue samples in 1.5 mL of RNA-Solv ® Reagent per 100

PolyATtract® mRNA Isolation Systems

PolyATtract ® mRNA Isolation Systems The PolyATtract ® mRNA Isolation Systems utilize Promega's MagneSphere ® technology to eliminate the need for oligo(dT) cellulose