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SOUTHERN BLOTTING OF GENOMIC DNA

plus HindIII); avoid plasmid-derived markers (1 kb ladder,etc.)since it might cross-hybridize with the probe! 2.Run gel: • Ioad samples onto agarose gel (typically 0.8 to 1.0% agarose in TBE,depending on desired size range). • run gel at maximum rate

RNA实验方法

.Particulates in unfiltered buffer, skin oils, nonspecific antibody binding, addition of too much probe, inadequate post-hybridization washing, improper wash temperature, and other factors can create background fog or spots on membranes.Blocking can reduce

The Basics: RNase Control

"), changing gloves after touching skin (e.g., your face), door knobs, and common surfaces, having a dedicated set of pipettors that are used solely for RNA work, using tips and tubes that are tested and guaranteed