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文献和实验Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan
Neoepitope antibodies recognize the newly created N or C terminus of protein degradation products but fail to recognize the same sequence of amino acids present in intact or undigested protein. Aggrecan neoepitope antibodies have been pivotal
)containing the TFC4 gene tagged with two copies of EE and one flag epitopes inserted at the HpaI site near the N-terminus of TFC4. For Extract preparation,follow the Hahn Lab Method "Yeast WCE using microfluidizer" with the following modifications
Purification of TFIIIC from yeast
)containing the TFC4 gene tagged with two copies of EE and one flag epitopes inserted at the HpaI site near the N-terminus of TFC4. For Extract preparation,follow the Hahn Lab Method "Yeast WCE using microfluidizer" with the following modifications
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