Novagen特约代理商|71491 Overnight E

Novagen公司推出无需IPTG的自动诱导大肠杆菌培养基

培养基 是基于F. William Studier博士的技术，使用了不同代谢成分提高细胞的生长密度，并自动诱导lac启动子开始转录。Overnight Express Instant TB Medium培养基现在提供三种不同的成分。作为一种粉末装TB培养基它可快速溶解在水中，并进行微波加热和高压灭菌处理。

详细BAC DNA Isolation

well shallow plate. Incubate at 37 deg C overnight in a static growth chamber without shaking to produce the "starter culture". Add 1.8 ml of TB medium to each well in the 96 deep well blocks using the Eppendorf repeater pipette. Using the Hydra96

详细BACDNAIsolation

a smear of colonies and inoculate 3 ml TB medium containing the appropriate antibiotic. Grow the culture for 8 hours, 37 degrees C, shaking at 250 rpm in the Floor Shaker to produce the "starter culture". Add 1.8 ml of TB medium