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文献和实验genomic DNA elute/ modified lysis buffer solution (15 minutes at R/T under constant motion);5.Following the 15 minute incubation the beads are captured using a magnetic bead separator (Invitrogen™) and washed 3 times with 2x wash buffer
Preparing a Selenomethionyl Protein
overnight. Day 2 To each liter M9 (1x conc.) add: 10mL 20% Glucose (sterile filtered or autoclaved) 2mL 1M MgSO4 (sterile filtered) 0.05mL 2M CaCl2 (sterile filtered) 0.1mL 0.5% (w/v) thiamine solution (sterile filtered) 1mL
the technique of embedding specimens in plastic
; tweezers to handle specimen; paper tent (for protecting solution from dust contamination); nail to be used as pointed probe, bottle of Bio-Plastic. Step 2a: Remove specimen from 100% glycerin and place on paper towels. Mop up as much glycerin
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